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viral vector

Retroviral vector

Customized R&D is available to meet client needs, with research-grade retroviral vectors produced in 500 mL increments. The company provides comprehensive CDMO solutions, encompassing antibody screening, vector construction, viral vector packaging, CAR-T cell preparation, and in vitro/in vivo CAR-T cell activity validation. Furthermore, the company offers CAR-specific retroviral vector products targeting various tumor targets, such as BCMA, CD19, CD38, CD22, and EGFR/EGFRV III, all capable of efficiently transducing human primary T cells. The CD38 CAR, in particular, is a fully human anti-CD38 scFv structure obtained through phage display scFv antibody library screening, possessing independent intellectual property rights.

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Lentiviral vector

Lentiviral vectors are viral vector systems modified from HIV-1 that can effectively integrate exogenous genes or exogenous shRNAs into the host chromosome, thereby achieving persistent expression of the target sequence. Lentivirals have become one of the commonly used vector forms for expressing exogenous genes or exogenous shRNAs and are gaining increasingly widespread application. CellValley can provide customized lentiviral vector services targeting various tumor targets, such as BCMA, CD19, CD38, CD22, EGFR/EGFRVIII, etc.

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Adeno-associated virus vector

Adeno-associated viruses (AAVs) are a class of simple, non-enveloped, single-stranded DNA-deficient viruses capable of infecting both dividing and non-dividing cells. They can package large genes, making them a preferred tool for gene overexpression. AAVs are characterized by good safety, a broad host cell range, low immunogenicity, and long-term expression of exogenous genes in vivo. Shenzhen Cell Valley provides adeno-associated virus vectors of various specifications and serotypes, including single-promoter single-gene, single-promoter multi-gene, and dual-promoter multi-gene subtypes.

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Non-viral vector

Non-viral vectors utilize the physicochemical properties of non-viral vector materials to mediate gene transfer. Shenzhen Cell Valley primarily provides its unique non-viral gene engineering technology (PiggyBac transposon modification), a core technology for CAR-T therapy development. PiggyBac can effectively deliver large amounts of genetic material to T cells, producing CAR-T products with a high percentage of stem memory cells. This includes various subtypes such as single-promoter single-gene, single-promoter multi-gene, and dual-promoter multi-gene.

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Adenovirus ADV

Adenovirus (ADV) is a non-enveloped, linear, double-stranded DNA virus with broad-spectrum cellular and tissue infectivity. Its infection process is vigorous, making it suitable for experiments involving high gene expression in a short time. Compared to other viruses, adenovirus has the advantage of longer insertion fragments and stronger expression activity. In vitro, adenovirus vectors exhibit high transduction efficiency (nearly 100%) and can transduce different cell types, making it an excellent tool for gene delivery to large numbers of difficult-to-transfect cells. However, in vivo, due to the strong immunogenicity and vigorous infection of adenovirus, it often induces local tissue inflammation and immune responses in animals, affecting the objectivity of animal signs and experimental results.

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Shenzhen Cell Valley Biopharmaceutical Co., Ltd. is a comprehensive one-stop outsourcing service provider in China focused on the cell and gene therapy industry. It is also one of the first CRO/CDMO companies in the country to possess GMP industrial production capabilities for clinical-grade retroviral vectors . The company is a major public technical service platform construction project for CRO/CDMO in Shenzhen and is included among the city's latest announced "20+8" strategic emerging industry projects.Shenzhen Cell Valley has the capability for standardized and industrialized production of GMP-grade cell products such as CAR-T cells. Its primary production lines include those for various cell products like CAR-T, CAR-NK, CAR-M, γδT, TIL, and TCR-T. Additionally, the company operates production lines for various viral vectors, including RVV, LVV, non-viral vectors, and AAV, as well as for cellular raw materials used in producing therapeutics such as exosomes, genetically engineered antibodies, cytokines, oncolytic viruses, and vaccines.

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viral vector